Dr. Anil Kumar Singh
Principal Scientist
anils13[at]gmail[dot]com/ anil[dot]singh12[at]icar[dot]gov[dot]in
Phone :
011-25841787 Ext. 213
Education :
Ph.D.: G.B. Pant University of Agriculture and Technology, Pantnagar Post-Doc: International Centre for Genetic Engineering and Biotechnology, New Delhi

Research Interest

My lab is interested in understanding the regulation of plant adaptation to changing environments using next-generation genomic and allied computational approaches. My lab is also interested in the identification and characterization of genes responsible for plant stress tolerance and their deployment using transgenic and gene editing approaches.

Awards & Honors

  • Elected Member of “Plant Tissue Culture Association-India” (2019)
  • Selected Member of “The National Academy of Sciences, India” (2017).
  • Delivered invited talk in 1st International Apple Symposium held at Yangling, Shaanxi, China during Oct. 10-16, 2016.
  • Delivered invited talk in 9th International Rosaceae Genomics Conference held at Nanjing, China during June. 26-30, 2018.
  • Delivered invited talk in 6th Plant Dormancy Symposium held at Kyoto, Japan during Oct. 23-26, 2018.
  • Travel Grant Award by Indian National Science Academy (INSA), New Delhi for attending and delivering invited talk in 9th International Rosaceae Genomics Conference at Nanjing, China.
  • Travel Grant Award by DST-Science and Engineering Research Board for attending and delivering invited talk in 6th Plant Dormancy Symposium at Kyoto, Japan.
  • Project grant award under “Scheme for Young Scientists” by Science and Engineering Research Board, Govt. of India.
  • Travel grant award by ICGEB, New Delhi for attending 6th International Rice Genetics Symposium at Manila, Philippines.


  1. Tanvi Sharma, NK Sharma, Prakash Kumar, Ganesh Panzade, Tanuja Rana, MK Swarnkar, Anil Kumar Singh, Dharam Singh, Ravi Shankar, Sanjay Kumar (2021) The first draft genome of Picrorhizakurrooa, an endangered medicinal herb from Himalaya. Scientific Reports 11: 14944.
  2. Ekta, Anil Kumar Singh*, D.M. Pandey (2021). Co-expression network analysis of acidic-responsive genes in Arabidopsis thaliana signifies hub genes expression and their key role assessment for acidity tolerance in Oryza sativa L. Biologia. DOI : 10.1007/s11756-021-00837-3.
  3. Ekta, Anil Kumar Singh, D.M. Pandey (2021) Microstructural changes in rice (Oryza sativa L.) leaves under varying low pH levels: A swept-source optical coherence tomography approach. Laser Physics. 31: 085601.
  4. Alok Ranjan, Ragini Sinha, S.K. Lal, S.K. Bishi, Anil Kumar Singh* (2021) Phytohormone signalling and cross-talk to alleviate aluminium toxicity in plants. Plant Cell Reports.DOI: 10.1007/s00299-021-02724-2.
  5. Alok Ranjan, Ragini Sinha, MeenuBala, Ashwani Pareek, S.L. Singla-Pareek, Anil Kumar Singh* (2021) Silicon-mediated abiotic and biotic stress mitigation in plants: Underlying mechanisms and potential for stress resilient agriculture. Plant Physiology and Biochemistry.163: 15-25.
  6. Alok Ranjan, Ragini Sinha, T.R. Sharma, A. Pattanayak, Anil Kumar Singh* (2021) Alleviating aluminum toxicity in plants: implications of reactive oxygen species signalling and crosstalk with other signaling pathways. Physiologia
  7. Hisayo Yamane, Anil Kumar Singh*, Janice E.K. Cooke (2021) Editorial: Plant Dormancy Research: From Environmental Control to Molecular Regulatory Networks. Tree Physiology.doi.10.1093/treephys/tpab035.
  8. YR Rao, MW Ansari, Anil Kumar Singh, N Bharti, Varsha Rani, Amit Verma, Ramwant Gupta, RK Sahoo, ZH Siddiqui, ZK Abbas, Gurdeep Bains, Brajendra, SK Guru, Randeep Rakwal, Narendra Tuteja, VR Kumar (2020) Ethylene mediated physiological response for in vitro development of salinity tolerant tomato. Journal of Plant Interactions. 15: 406-416.
  9. Rohit Joshi, Khirod Kumar Sahoo, Anil Kumar Singh, Khalid Anwar, R.K. Gautam, S.L. Krishnamurthy, S.K. Sopory, Ashwani Pareek, S.L. Singla-Pareek (2020) Enhancing trehalose biosynthesis improves yield potential in marker-free transgenic rice under drought, saline, and sodic conditions. Journal of Experimental Botany. 71: 653-668.
  10. Ekta, Anil Kumar Singh*, DM Pandey. 2020. Physiological, biochemical and microstructural changes in rice (Oryza sativa L.) under varying pH levels. BioRxiv. doi:10.1101/2020.08.17.253914.
  11. Monika Bhuria, Parul Goel, Sanjay Kumar, Anil Kumar Singh* (2019) Genome-wide identification and expression profiling of genes encoding universal stress proteins (USP) identify multi-stress responsive USP genes in Arabidopsis thaliana. Plant Physiology Reports24: 434-445.
  12. AmrinaShafi, InshaZahoor, Tejpal Gill, Paramvir Singh Ahuja, Sanjay Kumar, Anil Kumar Singh* (2019) Ectopic co-expression of the SOD and APX genes enhanced callus growth and in vitro regeneration in Arabidopsis. Plant Biotechnology Reports13: 273-283.
  13. AmrinaShafi, Tejpal Gill, Insha Zahoor, P.S. Ahuja, Y. Sreenivasulu, Sanjay Kumar, Anil Kumar Singh* (2019) Ectopic expression of SOD and APX genes in Arabidopsis alters metabolic pools and genes related to secondary cell wall cellulose biosynthesis and improve salt tolerance. Molecular Biology Reports46: 1985-2002.
  14. Vishal Sharma, Parul Goel, Sanjay Kumar, Anil Kumar Singh* (2019) An apple transcription factor, MdDREB76, confers salt and drought tolerance in transgenic tobacco by activating the expression of stress-responsive genes. Plant Cell Reports38: 221-241.
  15. Ragini Sinha, Tilak R. Sharma, Anil Kumar Singh* (2019) Validation of reference genes for qRT-PCR data normalisation in lentil (Lens culinaris) under leaf developmental stages and abiotic stresses. Physiology and Molecular Biology of Plants25: 123-134.
  16. SomDutt, Shruti Kirti, Tanmay Vaidya, Jai Parkash, Sanjeeta Kashyap, Nandini Sharma, Anil Kumar Singh (2018) External application of NADPH enhances biomass accumulation, seed germination and modulates expression of oxidative pentose phosphate pathway genes in Arabidopsis. Indian Journal of Plant Physiology 23: 748-759.
  17. Ragini Sinha, Awadhesh Kumar Pal, Anil Kumar Singh* (2018) Physiological, biochemical and molecular responses of lentil (Lens culinarisMedik.) genotypes under drought stress. Indian Journal of Plant Physiology23: 772-784.
  18. Parul Goel, Nitesh Kumar Sharma, Monika Bhuria, Vishal Sharma, Rohit Chauhan, ShivalikaPathania, Mohit K. Swarnkar, Vandna Chawla, Vishal Acharya, Ravi Shankar, Anil Kumar Singh* (2018)Transcriptome and co-expression network analyses identify key genes regulating nitrogen use efficiency in Brassica juncea L. Scientific Reports 8:7451.
  19. B.K. Gupta, K.K. Sahoo, Ajit Ghosh, A.K. Tripathi, Khalid Anwar, Priyanka Das, Anil Kumar Singh, Ashwani Pareek, S.K. Sopory, S.L. Singla-Pareek (2018) Manipulation of glyoxalase pathway confers tolerance to multiple stresses in rice. Plant, Cell & Environment41(5):1186-1200.
  20. Gopal Singh, Gagandeep Singh, Pradeep Singh, Rajni Parmar, Navgeet Paul, Radhika Vashist, M.K. Swarnkar, Ashok Kumar, Sanatsujat Singh, Anil Kumar Singh, Sanjay Kumar, R.K. Sharma (2017) Molecular dissection of transcriptional reprogramming of steviol glycosides synthesis in leaf tissue during developmental phase transitions in Stevia rebaudiana Bert. Scientific Reports.7: 11835.
  21. Poonam Sharma, GeetikaSirhindi, Anil Kumar Singh, Harpreet Kaur and Ruqia Mushtaq (2017) Consequences of copper treatment on pigeon pea photosynthesis, osmolytes and antioxindantsdefense. Physiology and Molecular Biology of Plants. 23:809-816.
  22. AmrinaShafi, A.K. Pal, Vishal Sharma, Sonika Kalia, Sanjay Kumar, P.S. Ahuja, Anil Kumar Singh* (2017) Transgenic potato plants overexpressing SOD and APX exhibit enhanced lignification and starch biosynthesis with improved salt stress tolerance. Plant Molecular Biology Reporter.35:504–518.
  23. Gulshan Kumar, Khushboo Gupta, ShivalikaPathania, M.K. Swarnkar, U.K. Rattan, Gagandeep Singh, R.K. Sharma, Anil Kumar Singh* (2017) Chilling affects phytohormone and post-embryonic development pathways during bud break and fruit set in apple (Malus domesticaBorkh.). Scientific Reports7: 42593.
  24. Monika Bhuria, Parul Goel, Sanjay Kumar, Anil Kumar Singh* (2016) The promoter of AtUSP is co-regulated by phytohormones and abiotic stresses in Arabidopsis thaliana. Frontiers in Plant Science. 7: 1957.
  25. Parul Goel, Monika Bhuria, Mamta Kaushal, Anil Kumar Singh* (2016) Carbon: Nitrogen interaction regulates expression of genes involved in N-uptake and assimilation in Brassica juncea L. PLoS One11(9): e0163061.
  26. Kuldip Jayaswal, Pallavi Mahajan, Gagandeep Singh, Rajni Parmar, Romit Seth, Aparnashree Raina, M.K. Swarnkar, Anil Kumar Singh, Ravi Shankar, R.K. Sharma (2016) Transcriptome analysis reveals candidate genes involved in blister blight defense in Tea (Camellia sinensis (L) Kuntze). Scientific Reports 6:30412.
  27. Hena Dhar, Mohit K. Swarnkar, Aditi Rana, Kanishak Kaushal, Anil Kumar Singh, R.C. Kasana, Arvind Gulati (2016) Complete genome sequence of a low temperature active and alkaline stable endoglucanase-producing Paenibacillus sp. Strain IHB B 3084 from the Indian Trans-Himalayas. Journal of Biotechnology.230:1-2.
  28. Gulshan Kumar, Usha Kumari Rattan, Anil Kumar Singh* (2016) Chilling-mediated DNA methylation changes during dormancy and its release reveal the importance of epigenetic regulation during winter dormancy in apple (Malus x domesticaBorkh.). PLoS One.11(2): e0149934.
  29. Gulshan Kumar, Preeti Arya, Khushboo Gupta, Vinay Randhawa, Vishal Acharya, Anil Kumar Singh* (2016)Comparative phylogenetic analysis and transcriptional profiling of MADS-box gene family identified DAM and FLC-like genes in apple (Malus x domestica). Scientific Reports.6: 20695.
  30. Rakshak Kumar, Dharam Singh, M.K. Swarnkar, Anil Kumar Singh, Sanjay Kumar (2016) Complete genome sequence of Arthrobacter alpinusERGS4:06, a yellow pigmented bacterium tolerant to cold and radiations isolated from Sikkim Himalaya. Journal of Biotechnology. 220:86-87.
  31. GeetikaSirhindi,Poonam Sharma, Preeti Arya, Parul Goel, Gulshan Kumar, Vishal Acharya, Anil Kumar Singh*(2016) Genome-wide characterization and expression profiling of TIFY gene family in pigeonpea (Cajanus cajan(L.) Millsp.) under copper stress. Journal of Plant Biochemistry and Biotechnology. 25: 301-310.
  32. Parul Goel, Anil Kumar Singh* (2015) Abiotic stresses downregulate key genes involved in nitrogen uptake and assimilation in Brassica juncea L. PLoS One 10(11): e0143645.
  33. Vinay Randhawa, Anil Kumar Singh, Vishal Acharya (2015) A systematic approach to prioritize drug targets using machine learning, a molecular descriptor- based classification model, and high-throughput screening of plant derived molecules: a case study in oral cancer. Molecular Biosystems 11:3362-3377.
  34. Gulshan Kumar, Anil Kumar Singh* (2015) Reference Gene validation for qRT-PCR based gene expression studies in different developmental stages and under biotic stress in apple. Scientia Horticulturae. 197: 597-606.
  35. Rakshak Kumar, Dharam Singh, M.K. Swarnkar, Anil Kumar Singh, Sanjay Kumar (2015) Complete genome sequence of Arthrobacter sp. ERGS1:01, a putative novel bacterium with prospective cold active industrial enzymes, isolated from East Rathong glacier in India. Journal of Biotechnology. 214:139-140.
  36. Shoib A. Baba, Tabasum Mohiuddin, Swaraj Basu, M.K. Swarnkar, A.H. Malik, Z.A. Wani, Nazia Abbas, Anil Kumar Singh*,Nasheeman Ashraf (2015) Comprehensive transcriptome analysis of Crocus sativus for discovery and expression of genes involved in apocarotenoid biosynthesis. BMC Genomics16:698.
  37. Jai Parkash, S. Kashyap, Shruti Kirti, Anil Kumar Singh*, SomDutt (2015) Cathepsin B cysteine protease gene is upregulated during leaf senescence and exhibits differential expression behaviour in response to phytohormones in PicrorhizakurrooaRoyle ex Benth. Plant Gene 3: 11-19.
  38. Anil Kumar Singh, Ritesh Kumar, A.K. Tripathi, B.K. Gupta, Ashwani Pareek, S.L. Singla-Pareek (2015) Genome-wide investigation and expression analysis of Sodium/Calcium exchanger gene family in rice and Arabidopsis. Rice8:21.
  39. AmrinaShafi, Rohit Chauhan, Tejpal Gill, M.K. Swarnkar, Y. Sreenivasulu, Sanjay Kumar, Neeraj Kumar, Ravi Shankar, P.S. Ahuja, Anil Kumar Singh* (2015) Expression of SOD and APX genes positively regulates secondary cell wall biosynthesis and promotes plant growth and yield in Arabidopsis under salt stress.Plant Molecular Biology 87: 615-631.
  40. AmrinaShafi, Tejpal Gill, Y. Sreenivasulu, Sanjay Kumar, P.S. Ahuja, Anil Kumar Singh* (2015). Improved callus induction, shoot regeneration, and salt stress tolerance in Arabidopsis overexpressing superoxide dismutase from Potentilla atrosanguinea. Protoplasma 252:41-51.
  41. Preeti Arya, Gulshan Kumar, Vishal Acharya, Anil Kumar Singh* (2014) Genome-wide identification and expression analysis of NBS-encoding genes in Malus x domestica and expansion of NBS genes family in Rosaceae. PLoS One 9(9): e107987.
  42. Jyoti Bhardwaj, Rohit Chauhan, M.K. Swarnkar, R.K. Chahota, Anil Kumar Singh, Ravi Shankar and S.K. Yadav (2013) Comprehensive transcriptomic study on horse gram (Macrotyloma uniflorum): De novo assembly, functional characterization and comparative analysis in relation to drought stress. BMC Genomics 14: 647.
  43. Anil Kumar Singh*, Vishal Sharma, A.K. Pal, Vishal Acharya and P.S. Ahuja (2013) Genome-wide organization and expression profiling of NAC transcription factor family in potato (Solanum tuberosum L.). DNA Research 20:403-423.
  44. P.K. Bhardwaj, Ritu Kapoor, Deep Mala, Geetika Bhagwat, Vishal Acharya, Anil Kumar Singh, S.K. Vats, P.S. Ahuja and Sanjay Kumar (2013) Braving the attitude of altitude: Caragana jubata at work in cold desert of Himalaya. Scientific Reports 3: 1022.
  45. Karan Acharya, A.K. Pal, Arvind Gulati, Sanjay Kumar, Anil Kumar Singh, P.S. Ahuja (2013) Overexpression of Camellia sinensis thaumatin-like protein, CsTLP in potato confers enhanced resistance to Macrophominaphaseolina and Phytophthora infestans infection. Molecular Biotechnology 54: 609-622.
  46. Anil Kumar Singh, Ritesh Kumar, A. Pareek, S.K. Sopory and S.L. Singla-Pareek (2012) Overexpression of rice CBS domain containing protein improves salinity, oxidative and heavy metal tolerance in transgenic tobacco. Molecular Biotechnology 52: 205-216.
  47. Ananda Mustafiz, Anil Kumar Singh, A. Pareek, S.K. Sopory and S.L. Singla-Pareek (2011) Genome-wide analysis of rice and Arabidopsis identifies two glyoxalase genes that are highly expressed in abiotic stresses. Functional & Integrative Genomics 11: 293-305.
  48. O.O. Obembe, T. Khan, J.O. Popoola, Anil Kumar Singh, R.C. Pant (2010) Endogenous levels of reducing sugars, free amino acids and phenols during various stages of in vitro culture of cotton (Gossypium Spp.). International Journal of Biological and Chemical Sciences 4 (6): 2371-2378.
  49. H.R. Kushwaha, Anil Kumar Singh, S.K. Sopory, S.L. Singla-Pareek, A. Pareek (2009) Genome-wide expression analysis of CBS domain containing proteins in Arabidopsis thaliana (L.) Heynh and Oryza sativa (L.) reveals their developmental and stress regulation. BMC Genomics 10: 200.
  50. Anil Kumar Singh, M.W. Ansari, A. Pareek, S.L. Singla-Pareek (2008) Raising salinity tolerant rice: recent progress and future perspectives. Physiology and Molecular Biology of Plants. 14: 137-154.
  51. Kanchan Nasare, Amit Yadav, Anil Kumar Singh, K.B. Shivasharanappa, Y.S. Nerkar, V.S. Reddy. (2007) Molecular and symptom analysis reveal the presence of new phytoplasma associated with sugarcane in India. Plant Disease 91: 1413-1418. (IF 4.438)
  52. Tanveer Khan, Anil Kumar Singh, R.C. Pant 2006. Regeneration via somatic embryogenesis and organogenesis in different cultivars of cotton (Gossypium spp.). In Vitro Cellular & Developmental Biology (Plants) 42: 498-501.
  53. Namita Singh, Anil Kumar Singh, V.K. Gupta, Surendra Kumar and R.C. Pant (2006) Uptake and translocation of iron in sugarcane (Saccharum officinarum L.) plants. Physiology and Molecular Biology of Plants 12(2): 181-183.
  54. M.W. Ansari, T.K. Nailwal, A. Gomathi, Anil Kumar Singh, G. Bains, A. Shukla, H.S. Chaube, U.S. Singh and R.C. Pant (2005) Mangiferine (1,3,6,7- tetra hydroxyxanthone C2-â–D-glucoside), a phenolic metabolite of mango (Mangifera indica L.), affects germination of spore of Fusarium sp. Journal of Plant Biology 32 (3): 1-5.
  55. Anil Kumar Singh, V.K. Gupta, Tanveer Khan, M.W. Ansari and R.C. Pant. (2004) Optimization of micropropagation protocol for sugarcane (Saccharum officinarum L.) cultivars. Plant Cell Biotechnology & Molecular Biology 5: 21-26.
  56. V.K. Gupta, Anil Kumar Singh, R.C. Pant. (2004) The effect of iron deficiency on root morphology, ethylene content and uptake of other micronutrients in in vitro grown cultivars of sugarcane (Saccharum officinarum L.). Indian Journal of Plant Physiology 9(2): 124-131.

PPublications in Genome Announcements (a journal of American Society of Microbiologists)


Madhu Kumari, Mohit K Swarnkar, Sanjay Kumar, Anil Kumar Singh*, Mahesh Gupta (2015) Genome sequence of a potential probiotic strain, Lactobacillus fermentum HFB3, isolated from a human gut. Genome Announcements3(6): e01296-15.

  1. Madhu Kumari, Mohit K Swarnkar, Sanjay Kumar, Anil Kumar Singh*, Mahesh Gupta (2015) Complete genome sequence of potential probiotic Lactobacillus sp. HFC8, isolated from human gut using PacBio SMRT sequencing. Genome Announcements 3(6): e01337-15.
  2. Rakshak Kumar, Dharam Singh, Mohit K Swarnkar, Anil Kumar Singh, Sanjay Kumar (2015) Genome assembly of Chryseobacteriumpolytrichastri ERMR1:04, a psychrotolerant bacterium with cold active proteases, isolated from East Rathong Glacier in India. Genome Announcements 3(6): e01305-15.
  3. Arvind Gulati, Mohit Kumar Swarnkar, Pratibha Vyas, Praveen Rahi, Rishu Thakur, Namika Thakur, Anil Kumar Singh (2015) Complete genome sequence of the rhizobacterium Pseudomonas trivialis strain IHBB745 with multiple plant growth-promoting activities and tolerance to desiccation and alkalinity. Genome Announcements 3(5): e00943-15.
  4. Shashi Kiran, Mohit K. Swarnkar, Mohinder Pal, Rishu Thakur, R. Tiwari, Anil Kumar Singh, Arvind Gulati (2015) Complete genome sequencing of a protease-producing novel Arthrobacter sp. strain IHBB 11108 using PacBio Single-Molecule Real-Time sequencing technology. Genome Announcements3(2):e00346-15.
  5. Mohinder Pal, Mohit K. Swarnkar, Rishu Thakur, Shashi Kiran, Sanjay Chhibber, Anil Kumar Singh, Arvind Gulati (2015) Complete Genome Sequence of Paenibacillus sp. Strain IHBB 10380 Using PacBio Single-Molecule Real-Time sequencing technology. Genome Announcements 3(2): e00356-15.
  6. Hena Dhar, MK Swarnkar, A Gulati, Anil Kumar Singh*, Ramesh C. Kasana (2015). Draft genome sequence of a cellulase-producing psychrotrophicPaenibacillusstrain, IHB B 3415, isolated from the cold environment of the western Himalayas, India. Genome Announcements 3(1): e01581-14.
  7. M.K. Swarnkar, Richa Salwan, R.C. Kasana, Anil Kumar Singh* (2014) Draft genome sequence of psychrotrophicAcinetobacter sp. strain MN12 (MTCC 10786), which produces a low-temperature-active and alkaline –stable peptidase. Genome Announcements 2(6): e01167-14.
  8. Richa Salwan, M.K. Swarnkar, Anil Kumar Singh*, R.C. Kasana (2014) First draft genome sequence of a member from the genus Planomicrobium, isolated from the Chandrariver, India. Genome Announcements 2(1): e01259-13.


Sneh Lata Singla-Pareek, Sudhir K Sopory, Ashwani Pareek and Anil Kumar Singh. A Method to enhance Multiple Abiotic Stress Tolerance of Crop Plants by over-expression of Cystathionine- β-synthase domain containing gene and Cystathionine-β-synthase domain containing gene therefor. (International Application Number PCT/IN2011/000140; Published by WIPO Sep. 15, 2011)


Page Updated On : 01/01/1970 - 00:00:00